CsLOX Gene Expression Analysis

Total RNA isolation was performed according to Wan et al. [46 (link)]. Gene-specific primers for RT–qPCR were designed using Primer 5, and the primer sequence is given in Supplementary Data Table S7. Gene expression analysis of CsLOXs was performed using SYBR Green in a BioRad IQ5 PCR thermal cycler (Bio-Rad, USA). The associations between CsLOX gene expression and alcohol content were investigated using Graphpad Prism 5.0 (GraphPad Software Inc., La Jolla, CA, USA).

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Sun Y., Li X., Ma Z, & Chen S. (2022). Quantitative trait locus mapping of fruit aroma compounds in cucumber (Cucumber sativus L.) based on a recombinant inbred line population. Horticulture Research, 9, uhac151.

Publication 2022

BioRad IQ5 PCR thermal cycler Graphpad Prism 5.0

Alcohol Gene Gene expression Gene expression analysis Isolation Primer Prism Sybr green

Corresponding Organization : Northwest A&F University

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Variable analysis

independent variables

CsLOX gene expression

dependent variables

Alcohol content

control variables

SYBR Green RT-qPCR in a BioRad IQ5 PCR thermal cycler

controls

Positive control: Not specified
Negative control: Not specified

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