Recombinant human MAO-A (hMAO-A) and MAO-B
(hMAO-B) were employed
for the MAO activity assay, with 0.06 mM kynuramine and 0.3 mM benzylamine
serving as the substrates, respectively.31 (link),32 (link) In this work, it was determined that the Km values for kynuramine for MAO-A and benzylamine for MAO-B
were 0.04–0.07 and 0.25–0.33 mM, respectively. Reference
MAO-A inhibitors included toloxatone and clorgyline, whereas reference
MAO-B inhibitors included lazabemide and pargyline. Enzymes, substrates,
and reference chemicals were purchased from Sigma-Aldrich (St. Louis,
MO, USA).
(hMAO-B) were employed
for the MAO activity assay, with 0.06 mM kynuramine and 0.3 mM benzylamine
serving as the substrates, respectively.31 (link),32 (link) In this work, it was determined that the Km values for kynuramine for MAO-A and benzylamine for MAO-B
were 0.04–0.07 and 0.25–0.33 mM, respectively. Reference
MAO-A inhibitors included toloxatone and clorgyline, whereas reference
MAO-B inhibitors included lazabemide and pargyline. Enzymes, substrates,
and reference chemicals were purchased from Sigma-Aldrich (St. Louis,
MO, USA).
