Confocal Microscopy Apoptosis Assay

Frozen sections at 6 μm were stained with a protocol established in our laboratory.^{44 (link), 45 (link)} Images were captured using a Zeiss 710 Meta Confocal Laser Scanning Microscope and analyzed using the Zeiss Zen 2010 software (Carl Zeiss Microscopy, LLC, Thornwood, NY). TUNEL assay was performed using TUNEL Apoptosis Detection kit (EMD Millipore, inc. Darmstadt, Germany) to determine cell apoptosis.

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He C., Lv X., Hua G., Lele S.M., Remmenga S., Dong J., Davis J.S, & Wang C. (2015). YAP forms autocrine loops with the ERBB pathway to regulate ovarian cancer initiation and progression. Oncogene, 34(50), 6040-6054.

Publication 2014

Zeiss 710 Meta Confocal Laser Scanning Microscope Zeiss Zen 2010 software TUNEL Apoptosis Detection kit

Apoptosis Assay Cell Confocal laser scanning microscope Frozen sections Microscopy Tunel

Corresponding Organization :

Other organizations : University of Nebraska Medical Center

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Variable analysis

independent variables

Frozen section thickness (6 μm)

dependent variables

Cell apoptosis (determined by TUNEL assay)

control variables

Staining protocol established in the laboratory
Imaging using Zeiss 710 Meta Confocal Laser Scanning Microscope
Image analysis using Zeiss Zen 2010 software

controls

Positive control: Not specified
Negative control: Not specified

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