Immunohistochemistry was carried out as previously described33 (link). The primary antibodies used were rabbit anti-GFP (1:1000; Invitrogen) and mouse nc82 (1:50, DSHB). The secondary antibodies used were goat anti-rabbit Alexa-488 and goat anti-mouse Alexa-568 (Invitrogen). Images are maximum intensity projections of confocal z-stacks acquired using a Leica SP5 II confocal microscope with 25x water immersion objective.
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Immunohistochemistry for GFP and nc82 Proteins
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Corresponding Organization :
Other organizations : University of British Columbia, University of California, Riverside
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- Primary antibodies used: rabbit anti-GFP (1:1000; Invitrogen) and mouse nc82 (1:50, DSHB)
- Immunohistochemistry results
- Immunohistochemistry protocol as previously described33 (link)
- Secondary antibodies used: goat anti-rabbit Alexa-488 and goat anti-mouse Alexa-568 (Invitrogen)
- Confocal z-stacks acquired using a Leica SP5 II confocal microscope with 25x water immersion objective
- No positive controls explicitly mentioned
- No negative controls explicitly mentioned
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