Details concerning the determination of the serum metabolome and the results related to feeding and parity are given in Pacífico et al. (4 (link)). Briefly, 10 μl of aliquots of serum samples were processed using a targeted metabolomics approach based on the Biocrates MxP® Quant 500 kit (Biocrates Life Sciences AG, Innsbruck, Austria). Analysis of serum metabolome as well as of reference standards and quality controls (provided by the manufacturer) was carried out by ultra-high-performance liquid chromatography (uHPLC) and flow injection analysis (FIA), both coupled to tandem mass spectrometry. An Agilent 1290 series UHPLC system coupled to a 6500+ QTrap mass spectrometer equipped with an Ion-Drive Turbo V® ESI source (both Sciex, Foster City, CA, USA) was used for the analysis. Chromatographic and mass spectrometric parameters were set according to manufacturer's instructions. Data analysis was carried out in Analyst 1.6.3 (Sciex) for LC-MS/MS data and in the Biocrates MetIDQ software for FIA-MS/MS data.
Free full text: Click here
