Mature sperm in the mouse cauda epididymis were prepared as described before.21 (link) Two caudal epididymis samples were placed into human tubal fluid (HTF) medium (cat# MR-070, Sigma–Aldrich). Then, we slightly cut the cauda of the epididymis into three pieces for incubation at 37°C for 5 min. We performed sperm concentration and motility analyses using the computer-assisted semen analysis system (CASA; WLJY-9000, Beijing Weili Co., Ltd., Beijing, China) according to the laboratory manual of the World Health Organization.3 Parameters including sperm concentration (×106 ml-1), rapid progressive motility (grade A; in %), progressive motility (grade A + B; in %), curve-line velocity (VCL; in μm s−1), straight-line velocity (VSL; in μm s−1), linearity (LIN; in %), average path velocity (VAP; in μm s−1), amplitude of lateral head displacement (ALH; in μm), and straightness (STR; in %) were evaluated. At least 200 sperm were counted for each assay. Seven, 8, 7, and 6 mice in the db/m, db/db, Dapa, and Ex groups were used for semen analysis, respectively.
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Sperm Characteristics in Diabetic Mice
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Other organizations : Peking University, Peking University Third Hospital
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Variable analysis
independent variables
- Mouse group (db/m, db/db, Dapa, Ex)
- Sperm concentration (×10^6 ml^-1)
- Rapid progressive motility (grade A; in %)
- Progressive motility (grade A + B; in %)
- Curve-line velocity (VCL; in μm s^-1)
- Straight-line velocity (VSL; in μm s^-1)
- Linearity (LIN; in %)
- Average path velocity (VAP; in μm s^-1)
- Amplitude of lateral head displacement (ALH; in μm)
- Straightness (STR; in %)
- Preparation of mature sperm from mouse cauda epididymis
- Incubation of sperm in human tubal fluid (HTF) medium at 37°C for 5 min
- Use of computer-assisted semen analysis (CASA) system according to World Health Organization guidelines
- No positive controls explicitly mentioned
- No negative controls explicitly mentioned
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