After transcardial perfusion using 3.7% paraformaldehyde, the maxillae were hemisected, and micro–focus computed tomography (µCT) scanning was performed using Siemens Inveon Micro-PET/SPECT/CT (Siemens) with 9 µm spatial resolution. The linear distance from the cementoenamel junction (CEJ) to the alveolar bone crest (ABC) on the buccal side was measured (Abe and Hajishengallis 2013 (link); Xiao et al. 2015 (link)).
Protocol detail
Quantifying Alveolar Bone Loss via μCT
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Alveolar crest
Bone
Cementoenamel junction
Computed tomography
Maxillae
Micro ct
Paraformaldehyde
Perfusion
Pet ct
Spect
Spect ct
Corresponding Organization :
Other organizations : University of Maryland, Baltimore
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Variable analysis
independent variables
- Transcardial perfusion using 3.7% paraformaldehyde
- Linear distance from the cementoenamel junction (CEJ) to the alveolar bone crest (ABC) on the buccal side
- Hemisected maxillae
- Micro–focus computed tomography (µCT) scanning with 9 µm spatial resolution using Siemens Inveon Micro-PET/SPECT/CT
- No positive or negative controls were explicitly mentioned in the provided information.
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