Immunoprecipitation of RAB3C and Dystrophin

We incubated the whole‐cell lysates (2 mg) from the cultured cells overnight in IP buffer with 25 μL of protein A/G magnetic beads and the corresponding antibodies against RAB3C (Cat # 15029‐1‐AP, Proteintech, Rosemont, IL, USA) or dystrophin (Cat # HPA023885, Atlas antibodies, Bromma, Sweden) in a 1.5‐mL microcentrifuge tube with a final volume of 1000 μL. We purified the proteins that interacted with the antibodies according to the manufacturer's protocol [38 (link)].

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Chang Y., Li C., Chan M., Fang C., Zhang Z., Chen C, & Hsiao M. (2023). Overexpression of synaptic vesicle protein Rab GTPase 3C promotes vesicular exocytosis and drug resistance in colorectal cancer cells. Molecular Oncology, 17(3), 422-444.

Publication 2023

Antibodies Buffer Cat 1 Cell Cultured cells Dystrophin G protein Protein a Protein g Proteins

Corresponding Organization :

Other organizations : National Yang Ming Chiao Tung University, Genomics Research Center, Academia Sinica, National Health Research Institutes, Taipei Medical University Hospital, National Taiwan University

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Variable analysis

independent variables

Whole-cell lysates (2 mg) from cultured cells
Antibodies against RAB3C (Cat # 15029-1-AP, Proteintech, Rosemont, IL, USA) or dystrophin (Cat # HPA023885, Atlas antibodies, Bromma, Sweden)

dependent variables

Proteins that interacted with the antibodies

control variables

Incubation overnight in IP buffer
Use of 25 μL of protein A/G magnetic beads
Final volume of 1000 μL

controls

No positive or negative controls were explicitly mentioned in the provided information.

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