Terpene Gene Cluster Characterization in Chrysosporium

The sequence of terpene type gene cluster (GME3317_g to GME3324_g) were acquired from the draft genome sequence of C. chrysosperma, which had been sequenced by our laboratory (NCBI GenBank accession number JAEQMF000000000). The genome sequences of the terpene type gene cluster and the corresponding CDS sequences were committed to the Gene Structure Display Server (GSDS)¹ to analyze the number and alignment of introns and exons. TBtools (V0.66836) was used to visualize the relative expression level (Chen et al., 2020 (link)). The homologs of this terpene type gene cluster were searched in the genome of other microorganisms in the JGI database. The domain structures of terpene type gene cluster were annotated using the InterProScan tool.² In addition, phylogenetic analysis was conducted with MEGA 10.0 software using the full-length protein sequences and neighbor-joining method with 1,000 bootstrap replications.

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Yang Y., Yu L., Qiu X., Xiong D, & Tian C. (2023). A putative terpene cyclase gene (CcPtc1) is required for fungal development and virulence in Cytospora chrysosperma. Frontiers in Microbiology, 14, 1084828.

Publication 2023

Exons Gene Gene cluster Gene structures Genome Introns Mega 10 Protein sequences Replications Terpene

Corresponding Organization : Beijing Forestry University

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Variable analysis

independent variables

Genome sequences of the terpene type gene cluster (from GME3317_g to GME3324_g) in the draft genome of C. chrysosperma
Homologs of the terpene type gene cluster in the genomes of other microorganisms

dependent variables

Number and alignment of introns and exons in the terpene type gene cluster
Relative expression level of the terpene type gene cluster
Domain structures of the terpene type gene cluster

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the information provided.

Annotations

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