Antibody Generation for UBTF, RPA194, and TAF1B

Rabbit polyclonal antibodies against mouse UBTF, RPI large subunit (RPA194/Polr1A), and TAF1B were generated in the laboratory and have been previously described [25 (link)], anti-TAF1C was a gift from I. Grummt. All other antibodies were obtained commercially; anti-Fibrillarin (#LS-C155047, LSBio), anti-RPA135 (#SC-293272, Santa Cruz), anti-RPA194 (#SC-48385, Santa Cruz), anti-TBP (#ab818 and #ab51841, Abcam) and anti-FLAG M2 (F3165, Sigma).

Free full text: Click here

Tremblay M.G., Sibai D.S., Valère M., Mars J.C., Lessard F., Hori R.T., Khan M.M., Stefanovsky V.Y., LeDoux M.S, & Moss T. (2022). Ribosomal DNA promoter recognition is determined in vivo by cooperation between UBTF1 and SL1 and is compromised in the UBTF-E210K neuroregression syndrome. PLoS Genetics, 18(2), e1009644.

Publication 2022

anti-RPA194 ab818 ab51841 anti-FLAG M2

Antibodies Fibrillarin Mouse Rabbit Subunit Ubtf

Corresponding Organization : Université Laval

Other organizations : University of Tennessee Health Science Center, University of Memphis

Top 5 similar protocols

Variable analysis

independent variables

Rabbit polyclonal antibodies against mouse UBTF
Rabbit polyclonal antibodies against RPI large subunit (RPA194/Polr1A)
Rabbit polyclonal antibodies against TAF1B
Anti-TAF1C antibody (a gift from I. Grummt)
Anti-Fibrillarin antibody (#LS-C155047, LSBio)
Anti-RPA135 antibody (#SC-293272, Santa Cruz)
Anti-RPA194 antibody (#SC-48385, Santa Cruz)
Anti-TBP antibodies (#ab818 and #ab51841, Abcam)
Anti-FLAG M2 antibody (F3165, Sigma)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!