Hyaluronic acid (60,000 Da, Genzyme Corporation, Cambridge, MA). Adipic dihydrazide, NHS-acrylate, acryloyl chloride, triethylamine, N-boc-ethylenediamine, 4-(4,6-dimethoxy[1 (link),3 (link),5 (link)]triazin-2-yl)-4-methylmorpholinium chloride (DMTMM), vanillin, potassium carbonate, ethyl-4-bromobutyrate, dimethylformamide (DMF), nitric acid, ethanol, sulfuric acid, trifluoroacetic acid, methanol, and acetic acid were purchased from Fisher Scientific and used without purification. Picoline borane complex and PEG dithiol (MW 1000 Da) were purchased from Sigma Aldrich and used without purification. Ac-FKGGGERC-NH2 (K peptide), Ac-GCRDGPQGIWGQDRCG-NH2, NQEQVSPLRGDSPGNH2 (Q-RGD peptide), and FITC-labeled NQEQVSPLRGDSPG-NH2 were purchased from Genscript (Piscataway, NJ) and used without purification. 4-arm PEG-vinylsulfone (PEG-VS, MW 40kDa) and 4-arm PEG-maleimide (PEG-MAL, MW 20kDa) were purchased from JenKem Inc. and used without further purification.
Protocol detail
Biomimetic Hydrogel Synthesis and Characterization
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Hyaluronic acid
acryloyl chloride
triethylamine
vanillin
potassium carbonate
dimethylformamide
nitric acid
ethanol
sulfuric acid
trifluoroacetic acid
methanol
acetic acid
Picoline borane complex
PEG dithiol
Ac-GCRDGPQGIWGQDRCG-NH2
Acetic acid
Acrylate
Acryloyl chloride
Adipic dihydrazide
Borane
Chloride
Dimethylformamide
Dithiol
Ethanol
Ethylenediamine
Fitc
Hyaluronic acid
Maleimide
Methanol
Nitric acid
Peptide
Picoline
Potassium carbonate
Rgd peptide
Sulfuric acid
Triazin
Triethylamine
Trifluoroacetic acid
Vanillin
Vinylsulfone
Corresponding Organization :
Other organizations : University of California, Los Angeles, Georgia Institute of Technology, Emory University
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Variable analysis
independent variables
- Hyaluronic acid (60,000 Da, Genzyme Corporation, Cambridge, MA)
- Adipic dihydrazide
- NHS-acrylate
- Acryloyl chloride
- Triethylamine
- N-boc-ethylenediamine
- 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methylmorpholinium chloride (DMTMM)
- Vanillin
- Potassium carbonate
- Ethyl-4-bromobutyrate
- Picoline borane complex
- PEG dithiol (MW 1000 Da)
- Ac-FKGGGERC-NH2 (K peptide)
- Ac-GCRDGPQGIWGQDRCG-NH2
- NQEQVSPLRGDSPGNH2 (Q-RGD peptide)
- FITC-labeled NQEQVSPLRGDSPG-NH2
- 4-arm PEG-vinylsulfone (PEG-VS, MW 40kDa)
- 4-arm PEG-maleimide (PEG-MAL, MW 20kDa)
- Not explicitly mentioned
- Dimethylformamide (DMF)
- Nitric acid
- Ethanol
- Sulfuric acid
- Trifluoroacetic acid
- Methanol
- Acetic acid
- Not explicitly mentioned
- Not explicitly mentioned
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