Porcine Kidney Macrophage Cell Line for ASFV

IPKM was established by immortalizing porcine primary culture of kidney macrophages with recombinant lentivirus vectors as previously described [20 (link)]. IPKM is highly susceptible to field ASFV isolates and cell-adapted ASFV isolates [21 (link),22 (link)]. The cells were routinely maintained in growth medium (Dulbecco’s modified Eagle’s medium (Nakali Tesque, Kyoto, Japan)) supplemented with 10% fetal bovine serum (FBS), 10 μg/mL bovine insulin (Merck, Darmstadt, Germany), 25 μM monothioglycerol (Wako, Osaka, Japan)), and antibiotics in cell culture plates and flasks for suspension culture (Sumitomo Bakelite, Tokyo, Japan). Porcine alveolar macrophage (PAM) cells were prepared from 8-week-old Landrace (L), Yorkshire (W), and Duroc (D) cross breed (LWD) pigs as described previously and stored at −80 °C.