BALF samples were cultured for aerobic bacteria on blood agar and Casmin (NAD-enriched) plates to indicate the presence of concurrent bacterial pneumonia. PCR assays for porcine circovirus 2 (PCV2) were conducted for swine BALF samples [31 (link)]. To exclude other causes of pneumonia in pigs, commercial assays for Mycoplasma hyopneumoniae and PRRSV were conducted according to the manufacturer’s recommendations (VetMax, Life Technologies, Carlsbad, CA, USA).
Souza C.K., Kimble J.B., Anderson T.K., Arendsee Z.W., Hufnagel D.E., Young K.M., Gauger P.C., Lewis N.S., Davis C.T., Thor S, & Vincent Baker A.L. (2023). Swine-to-Ferret Transmission of Antigenically Drifted Contemporary Swine H3N2 Influenza A Virus Is an Indicator of Zoonotic Risk to Humans. Viruses, 15(2), 331.
Corresponding Organization : National Animal Disease Center
Other organizations :
Iowa State University, University of London, Royal Veterinary College, Centers for Disease Control and Prevention, National Center for Immunization and Respiratory Diseases
Culturing BALF samples for aerobic bacteria on blood agar and Casmin (NAD-enriched) plates
Conducting PCR assays for porcine circovirus 2 (PCV2) on swine BALF samples
dependent variables
Presence of concurrent bacterial pneumonia
Presence of porcine circovirus 2 (PCV2) in swine BALF samples
control variables
Conducting commercial assays for Mycoplasma hyopneumoniae and PRRSV according to manufacturer's recommendations to exclude other causes of pneumonia in pigs
controls
Positive control: Not specified
Negative control: Not specified
Annotations
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