Serum neurofilament light chain (sNfL) and serum glial fibrillary acidic protein
(sGFAP) were measured using Simoa assays16 (link)–18 (link, link) (Quanterix; NF light
Simoa Assay Advantage Kit, GFAP Simoa Discovery Kit). All samples were analyzed
for the same target (sNFL or sGFAP) at the same time point. Samples were thawed
and processed as recommended by the manufacturer and previously described.19 (link)
We assessed the intra-assay coefficient of variation (CV) by testing a
quality control serum sample in five replicates. A CV of lower than 10% had to
be achieved for valid analysis. Concentrations were calculated using
corresponding standard curves. The laboratory personnel were blinded for
clinical data.
(sGFAP) were measured using Simoa assays16 (link)–
Simoa Assay Advantage Kit, GFAP Simoa Discovery Kit). All samples were analyzed
for the same target (sNFL or sGFAP) at the same time point. Samples were thawed
and processed as recommended by the manufacturer and previously described.19 (link)
We assessed the intra-assay coefficient of variation (CV) by testing a
quality control serum sample in five replicates. A CV of lower than 10% had to
be achieved for valid analysis. Concentrations were calculated using
corresponding standard curves. The laboratory personnel were blinded for
clinical data.
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