Protocol detail

Adiponectin Measurement in Large Cohorts

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Plasma adiponectin was measured among a subset of participants in the NHS and HPFS using the enzyme-linked immunosorbent assay (ALPCO Diagnostics, Salem, NH)^{31 (link), 33} or competitive radio-imunoassay (Linco Research Inc, St Charles, Mo)^{32 (link), 34 (link)}, as previously described. Quality-control samples were randomly interspersed and personnel who were blinded to quality-control status conducted all assays. The coefficients of variation from quality control samples ranged from 3.4–13.0%. To account for potential variation across batches, we corrected plasma adiponectin for measurement batch according to the average batch correction method with adjustment for age and body mass index (BMI)^{36 (link)}.

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Song M., Gong J., Giovannucci E.L., Berndt S.I., Brenner H., Chang-Claude J., Curtis K.R., Harrison T.A., Hoffmeister M., Hsu L., Jiao S., Le Marchand L., Potter J.D., Schoen R.E., Seminara D., Slattery M.L., White E., Wu K., Ogino S., Fuchs C.S., Hunter D.J., Tworoger S.S., Hu F.B., Rimm E., Jensen M., Peters U, & Chan A.T. (2014). Genetic variants of adiponectin and risk of colorectal cancer. International journal of cancer. Journal international du cancer, 137(1), 154-164.

Publication 2014

enzyme-linked immunosorbent assay

Adiponectin Assays Body mass index Diagnostics Enzyme linked immunosorbent assay Plasma

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Variable analysis

independent variables

Measurement batch

dependent variables

Plasma adiponectin

control variables

Body mass index (BMI)

controls

Quality-control samples were randomly interspersed and personnel who were blinded to quality-control status conducted all assays.

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