The secretome of esophageal myofibroblasts was studied after iTRAQ labelling of proteins in media followed by LC-MS/MS as previously described (see Methods S1) [3] (link). Putative chemerin targets in MSCs were sought after SILAC labelling of cells followed by exposure to chemerin (R&D Systems, Abindgon, Oxfordshire, UK) for 24 h and processing of cells for LC-MS/MS (see Methods S1) as previously described [23] (link).
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