Psoralen Modulation of Wnt/β-catenin Signaling

To further explore the effects of psoralen on the transcriptional activation of Wnt/β-catenin signaling pathway, western blot analysis was conducted according to the previous protocol^{20 (link)}. Using β-actin as an internal reference, the membrane was subsequently incubated at 4 °C overnight with primary antibodies against β-catenin, phospho-β-catenin and Fra-1 diluted at 1:3000, β-actin diluted at 1:5000 (Proteintech Group, CHI, USA), and enhanced chemiluminescence (ECL) plus kit (Millipore, America) was applied for visualization. The β-catenin expression and intracellular localization were analyzed using immunofluorescence. MDA-MB-231 cells and MCF-7 cells were fixed and stained with β-catenin antibodies (1:200 dilution). Images were captured by fluorescent microscopy.