Gels were Coomassie stained and scanned as described (Antrobus and Borner, 2011 (link)). Western blotting was performed as in Borner et al. (2012 (link)). Immunofluorescence microscopy was performed as described for Dmel2 cells (Hirst et al., 2009 (link)). The following antibodies were used: dAP1G (Hirst et al., 2009 (link)), dCLC (Heerssen et al., 2008 (link)), and anti-V5 (46-0705; Life Technologies). Images were adjusted for brightness and contrast in Photoshop (Adobe Systems Europe, Maidenhead, UK) or PowerPoint (Microsoft).
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Protein Detection Methods in Dmel2 Cells
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Other organizations : Max Planck Institute of Biochemistry, University of Cambridge
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Variable analysis
independent variables
- None explicitly mentioned
- Protein expression levels (measured by Coomassie staining and Western blotting)
- Protein localization (measured by immunofluorescence microscopy)
- None explicitly mentioned
- Positive control: Coomassie staining and Western blotting were performed as described in previous publications (Antrobus and Borner, 2011; Borner et al., 2012)
- Positive control: Immunofluorescence microscopy was performed as described for Dmel2 cells in a previous publication (Hirst et al., 2009)
- Antibody controls: The following antibodies were used: dAP1G (Hirst et al., 2009), dCLC (Heerssen et al., 2008), and anti-V5 (46-0705; Life Technologies)
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