Antibody Panel for Centrosome Research

Antibodies used in this study include polyclonal rabbit anti-Kif24 (ref. 21 (link)) (1:500 for western blot (WB) and 1:200 for immunofluorescence (IF)), anti-Nek2 (1:100 for WB and IF, cat. #610593, BD Biosciences), anti-Cep164 (1:2,000 for IF, gift from Dr Eva Lee and 1:5,000 for IF, cat. #45330002, Novus), anti-centrin-2 (1:1,000 for IF, cat. #04-1624, Millipore), β-actin (1:5,000 for WB, cat. #ab6276, Abcam), anti-α-tubulin (1:5,000 for WB, cat. #T5168), Flag (1:1,000 for WB and 1:200 for IF, cat. #F1804) and γ-tubulin (1:1,000 for IF, cat. #T6557, all from Sigma-Aldrich), anti-GFP (1:1,000 for WB, cat. #G1544, Sigma-Aldrich and 1:100 for WB, cat. #sc-8334, Santa Cruz Biotechnology), anti-glutamylated tubulin (GT335) (1:1,000 for IF, cat. #AG-20B-0020-C100, Adipogen), anti-IFT88 (1:500 for IF, cat. #13967-1-AP, Proteintech), anti-CPAP (1:500 for IF, gift from Dr Tang Tang), anti-Talpid3 (ref. 36 (link)) (1:500 for WB), anti-SAS6 (1:100 for IF, cat. #sc-81431), and Cyclin A (1:100 for WB, cat. #sc-596, all from Santa Cruz Biotechnology), anti-phospho-ser10-Histone H3 (1:1,000 for WB, cat. #06-570, Millipore), anti-Arl13b (1:100 for IF, gift from T. Katada and K. Kontani), and anti-Ki67 (1:1,000 for IF, cat. #ab15580, Abcam).