Western blots were carried out as previously described [25 (link)]. Primary antibodies for protein detection included: GLS1 (ab93434, Abcam), PARP1 (F-2, Santa Cruz), PAR (Trevigen, Gaithersburg, MD), Actin (C-2, Santa Cruz), small subunit calpain (EPR3324, Abcam), and 53BP1 (Bethyl Laboratories).
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