Characterization of Pycnoporus cinnabarinus Glyoxal Oxidases

All chemicals were of analytical grade. HMF, DFF, HMFCA, FFCA, FDCA, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and glyoxylic acid, together with the enzymes HRP and catalase were purchased from Sigma Aldrich (Lyon, France). The glyoxal oxidases from Pycnoporus cinnabarinus, PciGLOX1 and PciGLOX2, were heterologously produced in Aspergillus niger as described previously [25 (link)]. The same protocol was used to produce the third isoform, PciGLOX3, and is reported here for the first time. The aryl alcohol oxidase (AAO) from Ustilago maydis (UmaAAO) was produced as previously reported in our laboratory [36 (link)]. The sequences of PciGLOX1, PciGLOX2 and PciGLOX3 are available in GenBank under accession numbers KU215437, KU215438 and MK268804, respectively.