Tau Protein Labeling Antibodies

Monoclonal antibody AT8 (Thermo Scientific MN1020 - 1:400 for immunolabelling) recognises phosphorylated residues serine 202 and threonine 205 of Tau, and monoclonal antibody AT100 (Thermo Scientific MN1060 - 1:400 for immunolabelling) recognises phosphorylated residues threonine 212 and serine 214 of Tau. Tau C-ter is a polyclonal rabbit antibody, which recognises the carboxyl terminal region of Tau [23 (link)]. The monoclonal antibody MC1 was a generous gift from Peter Davis and recognises conformational changes in residues seven to nine and 313-322 (1:1000 for immunolabelling). ADx215 is a human specific anti-Tau antibody that recognizes Tau only when Tyr18 residue is dephosphorylated [4 (link)]. Mouse monoclonal (Invitrogen P/N-0705; 1:10000 for immunolabelling) and rabbit polyclonal antibodies (1:10000 for immunolabelling) to V5 recognise the V5 epitope of tagged Tau.

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Dujardin S., Lécolle K., Caillierez R., Bégard S., Zommer N., Lachaud C., Carrier S., Dufour N., Aurégan G., Winderickx J., Hantraye P., Déglon N., Colin M, & Buée L. (2014). Neuron-to-neuron wild-type Tau protein transfer through a trans-synaptic mechanism: relevance to sporadic tauopathies. Acta Neuropathologica Communications, 2, 14.

Publication 2014

AT100

Anti antibody Antibodies Antibody Epitope Human Monoclonal antibody Mouse Rabbit Serine Threonine

Corresponding Organization :

Other organizations : Inserm, CEA Paris-Saclay - Etablissement de Fontenay-aux-roses, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, KU Leuven, Université de Lille

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Variable analysis

independent variables

Monoclonal antibody AT8 (Thermo Scientific MN1020 - 1:400 for immunolabelling)
Monoclonal antibody AT100 (Thermo Scientific MN1060 - 1:400 for immunolabelling)
Tau C-ter polyclonal rabbit antibody
Monoclonal antibody MC1 (1:1000 for immunolabelling)
ADx215 human specific anti-Tau antibody
Mouse monoclonal (Invitrogen P/N-0705; 1:10000 for immunolabelling) and rabbit polyclonal antibodies (1:10000 for immunolabelling) to V5

dependent variables

Phosphorylated residues serine 202 and threonine 205 of Tau
Phosphorylated residues threonine 212 and serine 214 of Tau
Conformational changes in residues seven to nine and 313-322 of Tau
Tau when Tyr18 residue is dephosphorylated

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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