Diabetic Nephropathy Rat Model Induction

The DN rat model was induced according to our previously published study [17 (link)]. DN was induced in the overnight fasted rats by a one-time intraperitoneal injection of streptozotocin (STZ) (50 mg/kg, Sigma-Aldrich, St. Louis, MO, USA) dissolved in citric acid buffer 40 mg/mL (pH 4.5, Sigma, St. Louis, USA) [17 (link),24 (link),25 (link)]. Rat blood glucose was maintained at 350 mg/dL by injection with insulin (0.4 unit/rat) and maintained for 12 weeks to establish the DN rat model successfully [17 (link),26 (link),27 (link)].

Free full text: Click here

Hsiao C.C., Hou Y.S., Liu Y.H., Ko J.Y, & Lee C.T. (2021). Combined Melatonin and Extracorporeal Shock Wave Therapy Enhances Podocyte Protection and Ameliorates Kidney Function in a Diabetic Nephropathy Rat Model. Antioxidants, 10(5), 733.

Publication 2021

citric acid buffer

Blood glucose Buffer Citric acid Insulin Intraperitoneal injection Streptozotocin

Corresponding Organization :

Other organizations : Chang Gung University, Kaohsiung Chang Gung Memorial Hospital

Top 5 similar protocols

Variable analysis

independent variables

One-time intraperitoneal injection of streptozotocin (STZ) (50 mg/kg)

dependent variables

Rat blood glucose levels maintained at 350 mg/dL

control variables

Overnight fasting of rats
Citric acid buffer (40 mg/mL, pH 4.5) used to dissolve STZ
Insulin injection (0.4 unit/rat) to maintain blood glucose at 350 mg/dL for 12 weeks

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!