Evaluating rTs-MAPRC2-Ab Efficacy on Nematode Stages

At the ML and NBL stages, the selected concentration rTs-MAPRC2-Ab ratios (1:50, 1:200, and 1:800 dilutions) were used in conjunction with controls pET32a rat serum (control group) and PBS (control group). A 6-well plate was used with 2000 worms/well of both stages (ML, NBL). Culture medium was composed of RPMI-1640, 10% heat-inactivated FBS (Fetal Bovine Serum), and 2% antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin) (Gibco, Paisley, UK), and incubated at 37 °C with 5% CO₂. ML stage parasites were tested at various time intervals (0 h, 4 h, 8 h, 24 h) for motility and ecdysis (molting process) following methods in the literature [33 (link),34 (link),35 (link)]. NBL motility was checked after 24 h. An inverted bright field microscope (Olympus, Shibuya, Japan) was used to examine the phenotypic appearance at both stages (ML and NBL) [24 (link)].