Neutrophil Depletion in Glycerol-Induced Muscle Injury

For neutrophil depletion, mice were injected via the tail vein with 200 µg per mouse of a neutrophil-depleting antibody (anti-Gr1; BioLegend, clone RB6-8C5) or the isotype control (IgG2b; BioXCell, clone LTF-2) 24 h before glycerol-mediated injury of the TA muscle. Subsequently, these mice were further injected by i.p with another neutrophil-depleting antibody (anti-Ly6G; BioXCell, clone 1A8, 200 µg per mouse) or the corresponding isotype control (IgG2a; clone 2A3, 200 µg per mouse) 48 h and 96 h after the first dose of neutrophil-depleting antibody. BALB/c male mice (The Jackson Laboratory, JAX#000651) at 3 months of age were utilized for these experiments because anti-Gr1 and anti-Ly6G antibodies work better in this strain to deplete neutrophils^{73 (link),135 (link)–137 (link)}.

Free full text: Click here

Graca F.A., Stephan A., Minden-Birkenmaier B.A., Shirinifard A., Wang Y.D., Demontis F, & Labelle M. (2023). Platelet-derived chemokines promote skeletal muscle regeneration by guiding neutrophil recruitment to injured muscles. Nature Communications, 14, 2900.

Publication 2023

anti-Gr1 clone RB6-8C5 IgG2b clone LTF-2 anti-Ly6G clone 1A8 IgG2a BALB/c male mice

Anti antibodies Antibody Balb c mice Clone Glycerol Igg2a Igg2b Injury Isotype Male Mouse Muscle Neutrophil Strain Tail Vein

Corresponding Organization :

Other organizations : St. Jude Children's Research Hospital, Molecular Oncology (United States)

Top 5 similar protocols

Variable analysis

independent variables

Injection of neutrophil-depleting antibody (anti-Gr1, clone RB6-8C5) or isotype control (IgG2b, clone LTF-2) 24 h before glycerol-mediated injury of the TA muscle
Injection of additional neutrophil-depleting antibody (anti-Ly6G, clone 1A8) or corresponding isotype control (IgG2a, clone 2A3) 48 h and 96 h after the first dose of neutrophil-depleting antibody

dependent variables

Neutrophil depletion

control variables

Mouse strain (BALB/c male mice, 3 months of age)
Route of injection (tail vein and intraperitoneal)

positive controls

Isotype control antibodies (IgG2b and IgG2a)

negative controls

Mice not receiving neutrophil-depleting antibodies

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!