The H1_DL2 cell line used in this study is based on the H1 cell line isolated from a patient biopsy of human melanoma brain metastases as described previously [47 (link)]. The H1_DL2 cell line was developed by transducing H1 cells with two lentiviral vectors encoding Luciferase and a GFP variant of Dendra [37 (link)]. The cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (Gibco), 2% L-glutamine (Thermofisher Scientific), and MEM Non-Essential Amino Acids Solution (100X) (Thermofisher Scientific) diluted 1:25. The growth medium was exchanged twice a week.
Female NOD/SCID mice (eight weeks of age, 18–22 g of weight) were purchased from Harlan. The mice were housed in individually ventilated cages (Techniplast). In accordance with the recommendations set forth by the Federation of European Laboratory Animal Science Associations, animal housing conditions were free of specific pathogens. The mice were provided with sterile water and food ad libitum. All animal procedures were approved by the Norwegian National Animal Research Authorities, Mattilsynet,https://www.mattilsynet.no/ .
Female NOD/SCID mice (eight weeks of age, 18–22 g of weight) were purchased from Harlan. The mice were housed in individually ventilated cages (Techniplast). In accordance with the recommendations set forth by the Federation of European Laboratory Animal Science Associations, animal housing conditions were free of specific pathogens. The mice were provided with sterile water and food ad libitum. All animal procedures were approved by the Norwegian National Animal Research Authorities, Mattilsynet,
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