In Vitro Evaluation of Anti-Leukemic Compounds

Mouse p185⁺Arf^−/− B-ALL were grown in RPMI (Life Technologies, CA) with 10% fetal bovine serum, 55 μM 2-mercaptoethanol, 2 mM glutamine, penicillin, and streptomycin. Navitoclax (ABT-263), venetoclax (ABT-199), A-1155463, and A-1210477 were kindly provided by AbbVie, IL. EU-5346 (also known as Compound #9) was provided by Eutropic Pharmaceutical, MA [30 (link)]. UM-36 was kindly provided by Dr. Nikolovska-Coleska [28 (link), 29 (link)]. All compounds were solubilized in DMSO and added at indicated concentrations for cell culture assays.

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Koss B., Ryan J., Budhraja A., Szarama K., Yang X., Bathina M., Cardone M.H., Nikolovska-Coleska Z., Letai A, & Opferman J.T. (2016). Defining specificity and on-target activity of BH3-mimetics using engineered B-ALL cell lines. Oncotarget, 7(10), 11500-11511.

Publication 2016

RPMI Navitoclax venetoclax A-1155463 A-1210477

Abt 199 Abt 263 Assays Cell culture Dmso Fetal bovine serum Glutamine Mercaptoethanol Mouse Navitoclax Penicillin Pharmaceutical Streptomycin Venetoclax

Corresponding Organization : St. Jude Children's Research Hospital

Other organizations : Dana-Farber Cancer Institute, Harvard University, University of Tennessee Health Science Center, Eutropics Pharmaceuticals (United States), University of Michigan–Ann Arbor

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Navitoclax (ABT-263)
Venetoclax (ABT-199)
A-1155463
A-1210477
EU-5346 (also known as Compound #9)
UM-36

dependent variables

Cell culture assay outcomes

control variables

RPMI (Life Technologies, CA) with 10% fetal bovine serum, 55 μM 2-mercaptoethanol, 2 mM glutamine, penicillin, and streptomycin
All compounds were solubilized in DMSO and added at indicated concentrations for cell culture assays

positive controls

Not specified

negative controls

Not specified

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