High-throughput Nextera XT Library Prep

Sequencing libraries were prepared using the standard Illumina Nextera XT. DNA Sample Preparation kit (Illumina, #FC-131-1096) and the combination of 384 Combinatorial Dual Indexes (Illumina- Set A to D, #FC-131-2001 to FC-131-2004). Using the Mosquito liquid handling robot, the Nextera XT chemistry was miniaturized^{126 (link),127 (link)} (Supp. Dataset 10–14 and Supp. Information). All the final 384-pooled libraries were sequenced using Illumina HiSeq4000 NGS sequencer in a paired-end—150 bases length.

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Richter M.L., Deligiannis I.K., Yin K., Danese A., Lleshi E., Coupland P., Vallejos C.A., Matchett K.P., Henderson N.C., Colome-Tatche M, & Martinez-Jimenez C.P. (2021). Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy. Nature Communications, 12, 4264.

Publication 2021

Nextera XT DNA Sample Preparation kit Illumina- Set A to D HiSeq4000 NGS sequencer

Fc 131 Mosquito

Corresponding Organization : Ludwig-Maximilians-Universität München

Other organizations : Cancer Research UK, University of Cambridge, University of Edinburgh, MRC Institute of Genetics and Molecular Medicine, Western General Hospital, Centre for Inflammation Research, The Queen's Medical Research Institute

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Variable analysis

independent variables

Miniaturization of Nextera XT chemistry using Mosquito liquid handling robot

dependent variables

Sequencing of final 384-pooled libraries using Illumina HiSeq4000 NGS sequencer in a paired-end—150 bases length

control variables

Illumina Nextera XT DNA Sample Preparation kit (Illumina, #FC-131-1096)
Combination of 384 Combinatorial Dual Indexes (Illumina- Set A to D, #FC-131-2001 to FC-131-2004)

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