Vero E6 cells (originally ATCC CRL-1586, obtained from Cellonex in South Africa) were propagated in complete growth medium consisting of Dulbecco's modified Eagle medium with 10% fetal bovine serum (Hyclone) containing 10 mM HEPES, 1 mM sodium pyruvate, 2 mM l-glutamine and 0.1 mM nonessential amino acids (Sigma-Aldrich). Vero E6 cells were passaged every 3–4 days. The H1299-E3 cell line (H1299 originally from ATCC as CRL-5803) was propagated in growth medium consisting of complete Roswell Park Memorial Institute 1640 medium with 10% fetal bovine serum containing 10 mM HEPES, 1 mM sodium pyruvate, 2 mM l-glutamine and 0.1 mM nonessential amino acids. Cells were passaged every second day. The H1299-E3 (H1299-ACE2, clone E3) cell line was derived from H1299 as described in our previous work1 ,17 (link). Cell lines were not authenticated. Cell lines tested negative for mycoplasma contamination.
Other organizations :
Africa Health Research Institute, Centre for the AIDS Programme of Research in South Africa, Weizmann Institute of Science, National Health Laboratory Service, University of KwaZulu-Natal, South African Medical Research Council, University College London, Max Planck Institute for Infection Biology, University of Alabama at Birmingham, Stellenbosch University
Complete growth medium components (Dulbecco's modified Eagle medium with 10% fetal bovine serum, 10 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM nonessential amino acids) for Vero E6 cells
Complete Roswell Park Memorial Institute 1640 medium with 10% fetal bovine serum, 10 mM HEPES, 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM nonessential amino acids for H1299-E3 cells
Passage frequency (every 3-4 days for Vero E6 cells, every 2 days for H1299-E3 cells)
controls
Positive control: Not mentioned
Negative control: Not mentioned
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