Western Blot Analysis of Adipogenic Markers

Protein extractions were prepared with RIPA containing 1mM PMSF (Beyotime Institute of Biotechonogy, Haimen, China). Then the protein extractions were subjected to Western blot analysis, as previously described [38 (link),40 (link)]. Antibodies to FABP4 (Santa Cruz Biotechnology, sc-271529, 1:1000), Antibodies to PPARγ (Santa Cruz Biotechnology, sc-7196, 1:200), Antibodies to C/EBPa (Cell Signaling Technologies, #8178, 1:1000), and Anti-Tubulin (Beyotime Biotechnology, AT819, 1:2000) were used as primary antibodies. After being incubated with HRP-labeled goat anti-mouse IgG (Beyotime Biotechonogy, A0216, 1:1000) or anti-rabit IgG (Beyotime Biotechonogy, A0208, 1:1000), the blots were visualized using electrochemiluminescence (ECL; Millipore, Darmstadt, Germany). The band signal intensities in the western blots were quantified by ImageJ software (https://imagej.nih.gov/ij/).