Optical Tweezers Calibration Protocol

The optical tweezers (OT) system employed an infrared Ytterbium linearly polarized and collimated laser beam with a wavelength of 1064 nm and maximum power of 5 W (model YLR-5-1064-LP) (IPG Photonics, NY, USA). The laser was coupled to an inverted Nikon Eclipse TE300 microscope (Nikon, Melville, NY, USA) equipped with a PLAN APO 100X 1.4 NA DIC H Nikon objective, used to create the trap. The OT system was calibrated using the same procedure previously described [13 (link),25 (link),26 (link)]. The trap stiffness (k_OT) per unit power (P) at the objective entrance was

(0.25 \pm 0.01) pN μ m^{- 1} {mW}^{- 1} .

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Soares J., Araujo G.R., Santana C., Matias D., Moura-Neto V., Farina M., Frases S., Viana N.B., Romão L., Nussenzveig H.M, & Pontes B. (2020). Membrane Elastic Properties during Neural Precursor Cell Differentiation. Cells, 9(6), 1323.

Publication 2020

Nikon Eclipse TE300 microscope PLAN APO 100X 1.4 NA DIC H Nikon objective

Apo a 1 Microscope Ytterbium

Corresponding Organization : Universidade Federal do Rio de Janeiro

Other organizations : Instituto Estadual do Cérebro Paulo Niemeyer

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Variable analysis

independent variables

Laser power at the objective entrance

dependent variables

Trap stiffness per unit power at the objective entrance

control variables

Laser wavelength (1064 nm)
Laser type (infrared Ytterbium, linearly polarized and collimated)
Microscope (inverted Nikon Eclipse TE300)
Objective (PLAN APO 100X 1.4 NA DIC H Nikon)

controls

The OT system was calibrated using the same procedure previously described in references [13], [25], and [26].

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