Virus Propagation in Cell Lines

DENV (serotype 2, strain 16681) and ZIKV (strain BRA/Fortaleza/2015) were propagated in C6/36 and Vero cells, respectively^{46 (link), 47 (link)}. Encephalomyocarditis virus (EMCV, EMC strain) was purchased from ATCC and propagated in HEK293T cells^{6 (link)}. mutEMCV (EMCV-Zn_C19A/C22A), which carries two point mutations in the zinc domain of the L protein^{18 (link)}, was kindly provided by Frank J.M. van Kuppeveld (Utrecht University) and was propagated in BHK-21 cells. Sendai virus (strain Cantell) was purchased from Charles River Laboratories. SCoV2 (strain 2019-nCoV/USA_WA½020) was kindly provided by Jae U. Jung (Cleveland Clinic Lerner Research Center) and was propagated in Vero E6-hACE2 cells. All work relating to SCoV2 live virus and SCoV2-RNA was conducted in the BSL-3 facility of the Cleveland Clinic Florida Research and Innovation Center in accordance with institutional biosafety committee (IBC) regulations.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Liu G., Lee J.H., Parker Z.M., Acharya D., Chiang J.J., van Gent M., Riedl W., Davis-Gardner M.E., Wies E., Chiang C, & Gack M.U. (2021). ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity. Nature microbiology, 6(4), 467-478.

Publication 2021

Sendai virus

2019 ncov Cells Emcv Point mutations River Sendai virus Strain Vero cells Virus Zikv Zinc

Corresponding Organization :

Other organizations : Cleveland Clinic Florida, University of Chicago, Harvard University

Top 5 similar protocols

Protocol cited in 2 other protocols

Variable analysis

independent variables

Virus strains: DENV (serotype 2, strain 16681), ZIKV (strain BRA/Fortaleza/2015), EMCV (EMC strain), mutEMCV (EMCV-ZnC19A/C22A), Sendai virus (strain Cantell), SCoV2 (strain 2019-nCoV/USA_WA/2020)

dependent variables

Virus propagation in different cell lines: C6/36 cells, Vero cells, HEK293T cells, BHK-21 cells, Vero E6-hACE2 cells

control variables

Cell lines used for virus propagation
Biosafety level 3 (BSL-3) facility used for SCoV2 live virus and SCoV2-RNA experiments

positive controls

EMCV (EMC strain) obtained from ATCC

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!