Plasma Proteome Profiling Protocol

Collection, processing, and storage of the discovery and replication cohorts’ samples were performed according to study protocols from the HFGP (23 (link)). Venous whole-blood samples were collected using EDTA tubes and centrifuged into plasma before being stored at –80°C. Proteomic profiling was performed in plasma samples by Olink Proteomics AB using a proximity extension assay coupled with next-generation sequencing as a readout method (84 (link)). For this study, we used the Olink Explore 1536 platform, consisting of 1,472 proteins assigned into four 384-well multiplex panels focused on inflammation, cardiometabolic, oncologic, and neurologic proteins.

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Vadaq N., Zhang Y., Vos W.A., Groenendijk A.L., Blaauw M.J., van Eekeren L.E., Jacobs-Cleophas M., van de Wijer L., dos Santos J.C., Gasem M.H., Joosten L.A., Netea M.G., de Mast Q., Fu J., van der Ven A.J, & Matzaraki V. (2023). High-throughput proteomic analysis reveals systemic dysregulation in virally suppressed people living with HIV. JCI Insight, 8(11), e166166.

Publication 2023

Explore 1536

Assay Edta Inflammation Neurologic Oncologic Plasma Proteins Replication Venous blood

Corresponding Organization :

Other organizations : Radboud University Nijmegen, Radboud University Medical Center, University Medical Center Groningen, University of Groningen, Dr. Kariadi Hospital, Diponegoro University

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Variable analysis

independent variables

Collection, processing, and storage of the discovery and replication cohorts' samples were performed according to study protocols from the HFGP

dependent variables

Proteomic profiling was performed in plasma samples

control variables

Venous whole-blood samples were collected using EDTA tubes and centrifuged into plasma before being stored at –80°C
The Olink Explore 1536 platform, consisting of 1,472 proteins assigned into four 384-well multiplex panels focused on inflammation, cardiometabolic, oncologic, and neurologic proteins

controls

No positive or negative controls were explicitly mentioned in the provided information.

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