Quantitative PCR Analysis of Kidney and Liver

RNA was extracted from kidney and liver using Qiacube and the RNeasy Mini Kit (74106; Quiagen, Venlo, The Netherlands) as described previously [28 (link),33 (link)]. cDNA was synthesized using a Takara PrimeScript II 1st strand cDNA Synthesis Kit (6210; Takara Bio). Real-time PCR was performed using probes form Applied Biosystems, Waltham, MA, USA (β-actin Rn00667869_m1, Epo Rn00667869_m1, Hif2α Rn00576515_m1, Hif1α Rn01472831_m1, Phd2 Rn00710295_m1, Gr (Nr3c1) Rn00561369_m1, Rhcg Rn00788284_m1), and Premix Ex Taq (RP39LR; Takara Bio). mRNA expressions in control and IRI rats were compared by relative gene expression data using real-time quantitative PCR and the 2^−ΔΔCT.

Free full text: Click here

Yasuoka Y., Izumi Y., Fukuyama T., Oshima T., Yamazaki T., Uematsu T., Kobayashi N., Nanami M., Shimada Y., Nagaba Y., Mukoyama M., Sands J.M., Takahashi N., Kawahara K, & Nonoguchi H. (2024). Tubular Endogenous Erythropoietin Protects Renal Function against Ischemic Reperfusion Injury. International Journal of Molecular Sciences, 25(2), 1223.

Publication 2024

RNeasy Mini Kit Takara PrimeScript II 1st strand cDNA Synthesis Kit Premix Ex Taq

Corresponding Organization : Kitasato University Medical Center

Other organizations : Kitasato University, Kumamoto University, Hyogo University, Emory University

Top 5 similar protocols

Variable analysis

independent variables

Kidney and liver tissue samples

dependent variables

MRNA expressions of β-actin, Epo, Hif2α, Hif1α, Phd2, Gr (Nr3c1), and Rhcg

control variables

Positive control: Unknown
Negative control: Unknown

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!