Histological Bone Tissue Analysis

At postoperative week 8, bone tissue samples were removed and fixed with paraformaldehyde. Then, bone samples were completely decalcified in 15% EDTA solution. After decalcification, bone tissue samples were embedded in paraffin wax and cut into tissue sections. The tissue sections were stained with Masson trichrome, H&E and sirius red in accordance with standard protocols, and samples were observed using a light microscope and polarization microscope.

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Wang C., Dong J., Liu F., Liu N, & Li L. (2024). 3D-printed PCL@BG scaffold integrated with SDF-1α-loaded hydrogel for enhancing local treatment of bone defects. Journal of Biological Engineering, 18, 1.

Publication 2024

Corresponding Organization :

Other organizations : Shandong Provincial Hospital, Shandong First Medical University

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Variable analysis

independent variables

Postoperative week 8

dependent variables

Bone tissue structure and composition

control variables

Paraformaldehyde fixation
Decalcification in 15% EDTA solution
Paraffin embedding
Tissue sectioning
Staining with Masson trichrome, H&E, and sirius red

controls

No positive or negative controls were explicitly mentioned in the provided information.

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