JAKinibs and Venetoclax Administration in Mice

BALB/c and Rag2^−/− mice were purchased from Jackson Laboratory. All animal studies were performed according to NIH guidelines for the use and care of live animals and were approved by the NIAMS Institutional Animal Care and Use Committee. JAKinibs were resuspended in 0.5% methyl cellulose and animals were dosed orally twice daily with vehicle or 30 mg/kg of tofacitinib (kindly provided by Pfizer) or 20 mg/kg of PF-06651600 (provided by the National Center for Advancing Translational Sciences (NCATS), NIH) for 1 week (or 3 days, where indicated) (27 (link)). ABT-199 (Venetoclax, Selleckchem) was resuspended in 60% Phosal 50PG, 30% PEG 400, and 10% EtOH. Animals were dosed orally once a day with vehicle or 90 mg/kg for a week.