To investigate hyphal growth and cell aggregation, C. albicans cells were inoculated into 2 ml of PDB medium at a density of 105 CFU ml−1 in 14 ml test tubes with or without fatty acids (2 μg ml−1) or farnesol (100 μg ml−1) and incubated at 37°C for 24 h without shaking. After incubation for 24 h, aggregated cells and hyphal growths were visualized in bright field using the iRiSTM Digital Cell Imaging System (Logos Bio Systems) at magnifications of 4x and 10x. At least, four independent experiments were conducted.