Multiplex Immunoassay for Cytokine Analysis

The analysis of inflammatory markers has been described previously.^{25 (link)} In brief, a U-PLEX assay based on an electrochemiluminescent detection method (Meso Scale Diagnostics, Rockville, MD) was used to analyze the concentrations of 71 cytokines in plasma samples from patients (n = 13) with peripheral NeuP and healthy controls (n = 13). A MESO QUICKPLEX SQ 120 instrument equipped with DISCOVERY WORKBENCH data analysis software (Meso Scale Diagnostics, Rockville, MD) was used to collect and analyze data. Electrochemiluminescent signals from calibrators were fitted to a weighted 4-parametric logistic model to form standard curves.

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Jönsson M., Bäckryd E., Jonasson L., Gerdle B, & Ghafouri B. (2022). Differences in plasma lipoprotein profiles between patients with chronic peripheral neuropathic pain and healthy controls: an exploratory pilot study. Pain Reports, 7(5), e1036.

Publication 2022

U-PLEX assay MESO QUICKPLEX SQ 120 instrument DISCOVERY WORKBENCH data analysis software

Assay Cytokines Diagnostics Inflammatory Patients Plasma

Corresponding Organization : Linköping University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Concentrations of 71 cytokines in plasma samples from patients with peripheral NeuP and healthy controls

control variables

Patients with peripheral NeuP
Healthy controls

controls

Positive control: Calibrators used to form standard curves for electrochemiluminescent signals
Negative control: Not explicitly mentioned

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