Labeling Hair Cells and Macrophages

Hair cells were labeled with HCS-1, which is specific for otoferlin (Goodyear et al., 2010 (link)). HCS-1 was developed by Jeffrey Corwin (University of Virginia) and obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH, and maintained at the Department of Biology of the University of Iowa. HCS-1 was obtained as a purified concentrate and used at 1:500 dilution. The YFP or mCherry signals in macrophages were amplified by labeling with either anti-GFP or anti-mCherry (1:500; Thermo Fisher Scientific).

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Warchol M.E., Schrader A, & Sheets L. (2021). Macrophages Respond Rapidly to Ototoxic Injury of Lateral Line Hair Cells but Are Not Required for Hair Cell Regeneration. Frontiers in Cellular Neuroscience, 14, 613246.

Publication 2020

anti-GFP anti-mCherry

Corwin Dilution Hair cells Hybridoma Macrophages Otoferlin

Corresponding Organization : Washington University in St. Louis

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Variable analysis

independent variables

Labeling of hair cells with HCS-1, which is specific for otoferlin
Amplification of YFP or mCherry signals in macrophages by labeling with either anti-GFP or anti-mCherry

dependent variables

Observation of hair cells labeled with HCS-1
Observation of macrophages with amplified YFP or mCherry signals

control variables

Concentration of HCS-1 used (1:500 dilution)
Concentration of anti-GFP or anti-mCherry used (1:500)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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