Recombinant HIV-1 Tat Protein Production

Full length (101 amino acids) recombinant HIV-1 MN Tat was produced in the Escherichia coli expression system and purchased from ImmunoDiagnostics (Catalog. 1032; Woburn, MA, United States). CU-CPT22, OxPAPC, LPS, and Amlexanox were purchased from Sigma-Aldrich. CXCR3 antagonist AMG487 was obtained from Tocris Bioscience (Bristol, United Kingdom). The concentrations of these inhibitors were based on the concentration curve study and our previous reports (Niu et al., 2019 (link)). Cell Tracker Green CMFD was purchased from Invitrogen. Mouse CXCL10 Recombinant Protein was purchased from Invitrogen. Human CXCL10 Recombinant Protein and human CXCL10/IP-10 DuoSet Kit were obtained from R&D Systems (Minneapolis, MN, United States).

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Niu F., Liao K., Hu G., Moidunny S., Roy S, & Buch S. (2021). HIV Tat-Mediated Induction of Monocyte Transmigration Across the Blood–Brain Barrier: Role of Chemokine Receptor CXCR3. Frontiers in Cell and Developmental Biology, 9, 724970.

Publication 2021

CU-CPT22 LPS Amlexanox AMG487 human CXCL10/IP-10 DuoSet Kit

Amg487 Amino acids Amlexanox Cell tracker green Cu cpt22 Cxcr3 Escherichia coli Hiv tat Human Human cxcl10 protein Immunodiagnostics Inhibitors Mouse cxcl10 protein Oxpapc

Corresponding Organization : University of Nebraska Medical Center

Other organizations : Creighton University, Cedars-Sinai Smidt Heart Institute, Cedars-Sinai Medical Center, Sylvester Comprehensive Cancer Center, University of Miami

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Variable analysis

independent variables

Full length (101 amino acids) recombinant HIV-1 MN Tat
CU-CPT22
OxPAPC
Amlexanox
CXCR3 antagonist AMG487

dependent variables

Not explicitly mentioned.

control variables

The concentrations of these inhibitors were based on the concentration curve study and our previous reports (Niu et al., 2019 (link)).

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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