Protocol detail

Western Blot Analysis of Protein Profiles

Find Similar Protocols

Western blotting was carried out according to standard protocols (Ma et al., 2019 (link); Yang et al., 2020 (link)). Antibodies used in the western blot are listed as follows: anti-PAM (Abcam, ab237488), anti-SLC16A3 (Abcam, ab234728), anti-Stanniocalcin 1 (Abcam, ab124891), anti-CLDN9 (Proteintech, 16196-1-AP), anti-HDAC4 (Proteintech, 17449-1-AP), anti-KDELR3 (Proteintech, 27632-1-AP), anti-Versican (Abcam, ab19345), anti-triosephosphate isomerase (Abcam, ab170894), anti-CASP6 (Abcam, ab108335), anti-CHST6 (Abcam, ab154332), anti-N-Cadherin (Abcam, ab76011), anti-Vimentin (Abcam, ab92547), anti-PARP1 (Abcam, ab32138), anti-GAPDH (Proteintech, 60004-1-Ig), anti-Phospho-AKT (Proteintech, 66444-1-Ig), anti-p62 (Proteintech, 66184-1-Ig), Phospho-mTOR (Cell Signaling Technology, #5536), and anti-p-S6 Kinase (Cell Signaling Technology, #9209).

Free full text: Click here

Mou Z., Yang C., Zhang Z., Wu S., Xu C., Cheng Z., Dai X., Chen X., Ou Y, & Jiang H. (2020). Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. Frontiers in Genetics, 11, 566918.

Publication 2020

ab234728 ab19345 ab108335 ab76011 ab92547 ab32138 Phospho-mTOR

Antibodies Cadherin Casp6 Gapdh Mtor Parp1 S6 kinase Stanniocalcin 1 Triosephosphate isomerase Versican Vimentin Western blot

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein expression levels of PAM, SLC16A3, Stanniocalcin 1, CLDN9, HDAC4, KDELR3, Versican, triosephosphate isomerase, CASP6, CHST6, N-Cadherin, Vimentin, PARP1, Phospho-AKT, p62, Phospho-mTOR, and p-S6 Kinase

control variables

None explicitly mentioned

controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!