Liposarcoma Cell Line Validation

SW872 and SA-4 (both classified as “liposarcoma”) were purchased from ATCC. Pleomorphic liposarcoma cell lines: LiSa-2 was provided by P. Möller [14 (link)] and LS2 by D. Broccoli [15 (link)]. Cell lines from dedifferentiated liposarcomas: LPS141, LPS510 and LPS853 (all DDLPS) were received from J. Fletcher [16 (link)], GOT3 from Åman [17 (link)] and NRH-LS1 derived in-house [18 (link)]. Cell lines from well-differentiated liposarcomas: FU-DDLS-1, was provided by J. Nishio [19 (link)] and T449, T778 and T1000 were gifts from F. Pedeutour [20 (link)]. Cells were cultured in RPMI-1640 medium (#R0883) supplemented with 10% FBS, 1% L-Alanyl-L-Glutamine (all Sigma-Aldrich, St. Louis, USA) and grown at 37°C, 5% CO₂. Immortalized human mesenchymal stroma cells iMSC#3 [21 (link)] were cultured in minimum essential medium alpha medium (#32561029, ThermoFisher Scientific) with 20% FBS and L-Alanyl-L-Glutamine. Short tandem repeat (STR) DNA profiling was performed on all cell lines and their identity confirmed. Cells were confirmed negative for mycoplasma using the VenorGeM Mycoplasma Detection Kit (Minerva Biolabs, Berlin, Germany).