Kidney Morphology Analysis in Diabetic Mice

Animal protocols were approved and conducted in accordance with Yale Animal Resources Center and Institutional Animal Care and Use Committee regulations. Pdk1^fl/fl; Pax8^rtTA; TetO‐Cre mice and their induction regimen were previously described (Ma et al., 2013 (link)). Experiments were conducted on males. Retro‐orbital blood was collected from anesthetized mice for plasma BUN measurements (O’Brien Kidney Center at Yale). Upon sacrifice, kidney and body weights were recorded. Right kidneys were harvested and fixed in 4% PFA for histological analysis while contralateral kidneys were snap‐frozen. Images of whole‐kidney sections were obtained with a Nikon Eclipse TE2000‐U microscope under the control of MetaMorph software (Universal Imaging).