Desulfovibrio Bacteria Infection Effects

BMDMs untreated or infected with Desulfovibrio bacteria (Des-B) were cultured in DMEM (without phenol red; Gibco) without FBS for 6 h. Supernatants were collected and filtered with MILLEX-GP 0.22-μm filters (Merck Millipore Ltd., Co., Cork, Munster, Ireland) to remove cell debris, concentrated to 1% volume with Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa) (Merck Millipore Ltd., Co.) (Xie et al., 2015 (link); Jin et al., 2017 (link); Chen et al., 2020 (link)), and detected the concentrations of mouse-derived TNF-α with ELISA kit (#YFXEM00031, Yifeixue Biotechnology, Nanjing, China).

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Zhou J., Hou C., Chen H., Qin Z., Miao Z., Zhao J., Wang Q., Cui M., Xie C., Wang R., Li Q., Zuo G., Miao D, & Jin J. (2021). P16INK4a Deletion Ameliorates Damage of Intestinal Epithelial Barrier and Microbial Dysbiosis in a Stress-Induced Premature Senescence Model of Bmi-1 Deficiency. Frontiers in Cell and Developmental Biology, 9, 671564.

Publication 2021

DMEM MILLEX-GP 0.22-μm filters Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa)

Bacteria Cell Desulfovibrio Elisa Mouse Tnf α Ultrafiltration

Corresponding Organization : Nanjing Medical University

Other organizations : Plastic Surgery Hospital, Jiangsu Vocational College of Medicine

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Variable analysis

independent variables

Desulfovibrio bacteria (Des-B) infection

dependent variables

Concentration of mouse-derived TNF-α

control variables

Untreated BMDMs
Culture in DMEM (without phenol red; Gibco) without FBS for 6 h
Supernatant collection and filtration with MILLEX-GP 0.22-μm filters
Supernatant concentration to 1% volume with Amicon Ultra-4 centrifugal ultrafiltration tubes (NMWL 3KDa)

controls

Untreated BMDMs as negative control

Annotations

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