Analysis of RECQ4 and APC Complexes

Primary antibodies used were rabbit anti-RECQ4 (4-11) generated against residues 71–80 of human RECQ4 (WB 1:1000)^{10 (link)}, rabbit anti-RECQ4 (17008-1-AP, Proteintech, IP 1:200), mouse anti-RECQ4 (sc-518189, Santa Cruz, WB 1:1000), mouse anti-alpha tubulin (sc-5286, Santa Cruz, WB 1:1000), rabbit anti-APC1 (21748-1-AP, Proteintech, WB 1:1000), rabbit anti-APC5 (AP7109, Abclonal, WB 1:1000), mouse anti-APC11 (sc-517142, Santa Cruz, WB 1:1000), rabbit anti-beta actin (20536-1-AP, Proteintech, WB 1:1000), rabbit anti-BUB3 (27073-1-AP, Proteintech, WB 1:1000), mouse anti-BUB3 (sc-376506, Santa Cruz, WB 1:1000), rabbit anti-C1QBP (P32) (5734S, Cell Signaling, WB 1:1000), rabbit anti-CDC20 (10252-1-AP, Proteintech, WB 1:1000), rabbit anti-CDC45 (A2047, Abclonal, WB 1:1000), mouse anti-CDC6 (sc-9964, Santa Cruz, WB 1:1000), rabbit anti-CDT1 (A16576, Abclonal, WB 1:1000), rabbit anti-Cyclin A (PA5-16519, Thermo Fisher Scientific, WB 1:1000), mouse anti-Cyclin E (ab3927, Abcam, WB 1:1000), rabbit anti-DNA pol δ (sc-10784, Santa Cruz, WB 1:1000), rabbit anti-FBXO5 (EMI1) (10872-1-AP, Proteintech, WB 1:1000), mouse anti-FLAG (66008-3-Ig, Proteintech, WB 1:1000), rabbit anti-FLAG (20543-1-AP, Proteintech, WB 1:3000), rabbit anti-FZR1 (CDH1) (16368-1-AP, Proteintech, WB 1:1000), rabbit anti-Geminin (10802-1-AP, Proteintech, WB 1:1000), rabbit anti-GINS4 (A8592, Abclonal, WB 1:1000), rabbit anti-Histone H3 (sc-10809, Santa Cruz, WB 1:1000), rabbit anti-Lamin A/C (sc-20681, Santa Cruz, WB 1:1000), rabbit anti-MCM7 (ab52489, Abcam, WB 1:1000), rabbit anti-MCM2 (10513-1-AP, Proteintech, WB 1:1000), mouse anti-MCM5 (sc-165994, Santa Cruz, WB 1:1000), rabbit anti-ORC2 (A302-734A, Bethyl, WB 1:1000), rabbit anti-PSF2 (GINS2) (16247-1-AP, Proteintech, WB 1:1000), mouse anti-GINS2 (sc-376595, Santa Cruz, WB 1:1000), rabbit anti-PP2A (2039S, Cell Signaling, WB 1:1000), rabbit anti-SKP2 (A302-436A, Bethyl, WB 1:1000), rabbit anti-UBE1 (UBA1) (671981-1-Ig, Proteintech, WB 1:1000), rat anti-BrdU/CldU (MCA2060T, Bio-Rad, IF 1:200), mouse anti-BrdU/IdU (347580, BD Biosciences, IF 1:200), goat anti-rat IgG (H + L) Alexa Fluor Plus 488 conjugated (A48262, Invitrogen, IF 1:200), goat anti-mouse IgG (H + L) Alexa Fluor 568 conjugated (A-11004, Invitrogen, IF 1:200), goat anti-rat IgG (H + L) DyLight 488 (SA5-10018, Invitrogen, Flow Cytometry 1:100).

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Xu X., Chang C.W., Li M., Omabe K., Le N., Chen Y.H., Liang F, & Liu Y. (2023). DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation. Nature Communications, 14, 1233.

Publication 2023

Alexa 568 Alexa fluor 488 Alpha tubulin Anti dna Anti igg Antibodies Beta actin Brdu Cdh1 Cyclin a Cyclin e Fbxo5 Flow cytometry Fzr1 Geminin Goat Histone h3 Human Lamin a c Mcm2 Mcm5 Mouse Orc2 Pp2a Rabbit Recq4 Skp2 Uba1

Corresponding Organization :

Other organizations : Thermo Fisher Scientific (United States), City of Hope, University of Southern California

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

None explicitly mentioned

control variables

None explicitly mentioned

controls

Rabbit anti-RECQ4 (4-11) generated against residues 71–80 of human RECQ4 (used as a positive control, WB 1:1000)
Mouse anti-alpha tubulin (used as a loading control, WB 1:1000)

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