The method used to determine faecal SCFA levels has been described in detail previously[25 (link)]. Briefly, the faecal samples were homogenized with a solution containing 3 mmol/L 2-ethylbutyric acid and 0.5 mmol/L H2SO4. The homogenate was vacuum distilled, and the SCFA levels were determined by gas chromatography (Agilent 7890 A, Agilent, CA, United States) using a capillary column (serial no. USE400345H, Agilent J&W GC columns, Agilent) and flame ionization[35 (link),36 (link)] levels of total SCFAs, acetic, propionic, iso-butyric, n-butyric, iso-valeric, n-valeric acid, isocapronic and n-capronic acids, were determined and were expressed in units of mmol/kg wet weight.
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Quantifying Fecal Short-Chain Fatty Acids
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Acetic
Acid
Capillary
Capronic acids
Faecal
Flame ionization
Gas chromatography
N butyric acid
Vacuum
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Variable analysis
dependent variables
- Levels of total SCFAs
- Levels of acetic acid
- Levels of propionic acid
- Levels of iso-butyric acid
- Levels of n-butyric acid
- Levels of iso-valeric acid
- Levels of n-valeric acid
- Levels of isocapronic acid
- Levels of n-capronic acid
- Homogenization of fecal samples with a solution containing 3 mmol/L 2-ethylbutyric acid and 0.5 mmol/L H2SO4
- Vacuum distillation of the homogenate
- Gas chromatography (Agilent 7890 A, Agilent, CA, United States) using a capillary column (serial no. USE400345H, Agilent J&W GC columns, Agilent) and flame ionization for SCFA analysis
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