Enzymatic Activity Assay for Sortase A

The enzymatic activity of sortase A from Staphylococcus aureus was determined using SensoLyte 520 Sortase A Activity Assay Kit * Fluorimetric * (AnaSpec AS-72229, AnaSpec, San Jose, CA, USA) in accordance with the manufacturer’s instructions. DMSO at a concentration of 0.8% was used as a control. Fluorescence was measured with the plate reader PHERAStar FS (BMG Labtech, Offenburg, Germany) for 60 min with a time interval of 5 min. The data were processed by MARS Data Analysis v. 3.01R2 (BMG Labtech, Offen-burg, Germany). The results were presented as relative fluorescent units (RFUs) and percentage of the control data [28 (link)].