As previously described [17 (link)], terminal tumor tissue was stored in a 10% neutral buffered formalin for fixation and subsequent paraffin embedment. Formalin-fixed paraffin-embedded tumors were sectioned and stained with hematoxylin (Vector Laboratories, Newark, CA, USA, H-3401) and eosin (Harleco, Darmstadt, Germany, 200-12) to evaluate tissue morphology. Images were taken using an inverted microscope (Fisherbrand, Waltham, MA, USA, 03-000-013) with a digital camera attachment (Amscope, Irvine, CA, USA, MU503) at 40× magnification. Scale bars: 50 µm.
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