Behavioral and Amino Acid Analysis in Rats

All data are expressed as mean with SEM. Body weight was analyzed by a repeated-measures three-way ANOVA. The number of center zone entries and total distance in the OFT, the total duration of immobility in the FST, and free amino acid concentrations were analyzed using three-way ANOVA; significance was set at p < 0.05. Multiple regression analysis was applied to the OFT and FST for the variables of parental strain and sex of pups (strain: WIS rat =1, WKY rat = 2, sex: male =1, female = 2). All analyses were performed with StatView (version 5, SAS Institute Cary, USA, SAS, 1998). Outlying data from the behavioral tests and amino acid analysis were eliminated using Thompson’s test criterion for outlying observations (p < 0.01). Although several combinations of interactions were determined, only interactions indicating significant effects are provided in the relevant tables and figures.

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Ihara T., Hamada M, & Furuse M. (2023). The Greater Impact of Paternal, Compared to Maternal, Hereditary Background on Depressive-Like Behavior in Wistar Kyoto Rats with Different Amino Acid Metabolism in the Pup Brain. International Journal of Molecular Sciences, 24(4), 4199.

Publication 2023

StatView

Amino acid Anova Behavioral tests Body weight Female Male Parental Strain Wky rat

Corresponding Organization : Kyushu University

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Variable analysis

independent variables

Parental strain (WIS rat = 1, WKY rat = 2)
Sex of pups (male = 1, female = 2)

dependent variables

Body weight
Number of center zone entries in the Open Field Test (OFT)
Total distance in the OFT
Total duration of immobility in the Forced Swim Test (FST)
Free amino acid concentrations

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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