Gastrin-regulated Cell Migration Assay

The xCELLigence® DP system (Roche Diagnostics GmbH, Germany) was used to study migration as previously described [25 (link)]. Briefly, AGS-Gr cells were seeded into (5.0 x 10⁴ cells/well) the CIM-Plate 16 (Roche). The lower chamber contained 1 nM gastrin alone or in combination with ULK1 inhibitor SBI-0206965 (10 μM) final concentration) (Apex Biosciences A8715), Compound C (Millipore), HCQ (20 μM), BafA1 (100nM). Cell migration was monitored every 15 min on a RTCA DP instrument for 24 h. Data analysis was carried out using RTCA Software 1.2 supplied with the instrument.

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Rao S.V., Solum G., Niederdorfer B., Nørsett K.G., Bjørkøy G, & Thommesen L. (2017). Gastrin activates autophagy and increases migration and survival of gastric adenocarcinoma cells. BMC Cancer, 17, 68.

Publication 2017

xCELLigence® DP system CIM-Plate 16 Compound C

Cell migration Cells Diagnostics Gastrin Rtca Sbi 0206965 Ulk1

Corresponding Organization : Norwegian University of Science and Technology

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Variable analysis

independent variables

Gastrin (1 nM)
ULK1 inhibitor SBI-0206965 (10 μM)
Compound C
HCQ (20 μM)
BafA1 (100 nM)

dependent variables

Cell migration

control variables

AGS-Gr cells (5.0 x 10^4 cells/well)

controls

Positive control: Gastrin (1 nM)
Negative controls: ULK1 inhibitor SBI-0206965 (10 μM), Compound C, HCQ (20 μM), BafA1 (100 nM)

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